Season Two consists of 22 episodes that aired on Thursdays at 9:00 pm in the United States beginning September 28, 2006, and ending May 17, 2007.^[107]

The season follows Sam and Dean as they deal with their father's death and continue to hunt the yellow-eyed demon with assistance from new allies Ellen, Jo, and Ash. Part of the demon's master plan is eventually revealed as he gathers Sam and others like him, leading to Sam dying. Dean makes a deal with a crossroads demon to bring back Sam in exchange for his soul, which will be collected in one year. In a final confrontation, in which Dean kills the yellow-eyed demon, a hell's gate is opened, releasing numerous demons from within.